The film, a sequel to 2018's Spider-Man: Into The Spider-Verse, sees the pair embark on a life-altering journey across the multiverse that proves to be a rollercoaster ride from start to finish.
Talking about the prospect of exploring more of her character in the sequel, which delves deeper into Gwen's story and background, Steinfeld told RadioTimes.com: “It’s very exciting, it’s so fun! When I found out, just as a fan of the character myself, I was very excited.
"And to dig a little deeper into her world... It’s a space that I would live in forever, it is so beautiful and what they’ve done with it is amazing. We get a little bit more of Gwen this time around, which is fun.”
Fans will be thrilled to see the return of plenty of other familiar faces from the first film including Jake Johnson as Peter B Parker and Brian Tyree Henry as Morales’s father, Jefferson Davis, while new additions to the Spider-Verse include Jason Schwartzman as the movie’s villain, The Spot.
But who else is in the film's cast and who do they all play? Here's everything you need to know about the cast of Spider-Man: Across the Spider-Verse.
Spider-Man: Across the Spider-Verse cast - Full list of characters
The following cast members are confirmed to provide their voice talents for Spider-Man: Across the Spider-Verse.
- Shameik Moore as Miles Morales / Spider-Man
- Hailee Steinfeld as Gwen Stacy / Spider-Woman
- Brian Tyree Henry as Jefferson Davis
- Luna Lauren Vélez as Rio Morales
- Jake Johnson as Peter B Parker / Spider-Man
- Jason Schwartzman as Jonathan Ohnn / the Spot
- Issa Rae as Jessica Drew / Spider-Woman
- Karan Soni as Pavitr Prabhakar / Spider-Man India
- Daniel Kaluuya as Hobart "Hobie" Brown / Spider-Punk
- Oscar Isaac as Miguel O'Hara / Spider-Man 2099
- Greta Lee as Lyla
- Rachel Dratch as the school counsellor
- Jorma Taccone as Vulture
- Shea Whigham as George Stacy
- Andy Samberg as Ben Reilly / Scarlet Spider
Now, here is all you need to know about the major players in the film.
Shameik Moore as Miles Morales/Spider-Man
Of course, Shameik Moore is returning as Miles Morales, a character he first played in the widely-praised Into the Spider-Verse.
Chatting about the film at CinemaCon, Shameik explained: "It's been over a year after the events of the first move. Miles is still trying to figure out how to be a superhero.
"In the first film he learned that anyone can wear the mask, and in this film, Miles discovers that it's how you wear the mask that really makes you a hero. And the only person who really understands what he's going through is, of course, Gwen Stacy."
Where have I seen Shameik Moore before? Aside from voicing Miles, Moore is also known for his role as Malcolm in the 2016 film Dope, and for starring in the Netflix series The Get Down. Moore has also voiced his desire to play Miles in live-action.
Jason Schwartzman as Jonathan Ohnn / the Spot
A newcomer to across the Spider-Verse is Jason Schwartzman, who's set to voice villain the Spot.
Where have I seen Jason Schwartzman before? Schwartzman is known for roles in many Wes Anderson films including Rushmore, Fantastic Mr Fox, Moonrise Kingdom and The Grand Budapest Hotel.
Jake Johnson as Peter B Parker / Spider-Man
Jake Johnson will have a big role to play in Across the Spider-Verse as he returns as Peter B Parker, the Spider-Man who trained Miles. The last we saw of him, he'd made it back to his reality and was about to make amends with his ex-wife. But with the multiverse thrown into the mix, anything could happen.
Where have I seen Jake Johnson before? Johnson is best known for his role as Nick Miller in sitcom New Girl. He's also appeared in films like Get Him to the Greek and Jurassic World.
Hailee Steinfeld as Gwen Stacy / Spider-Woman
How can you have Miles Morales without Gwen Stacy? Hailee Steinfeld is back in her role because we absolutely cannot leave all the Spider-Man duties to the men. A new clip saw Miles and Gwen swinging through the streets of New York City and it's clear she's going to have an even bigger role to play in this film.
Where have I seen Hailee Steinfeld before? Steinfeld is known for her acting and her music career. She starred in 2010 Western True Grit, which earned her an Oscar nomination, and has appeared in the Pitch Perfect films, as well as The Edge of Seventeen. Most recently, she's played Emily Dickinson in Dickinson and Kate Bishop in Disney Plus's Hawkeye.
Issa Rae as Jessica Drew / Spider-Woman
Another new voice this time around will be Issa Rae as Spider-Woman - perhaps the most badass new character of them all. In the trailers, she's seen taking absolutely no nonsense and kicking into gear to battle Vulture, as she crashes through universes.
More like this
Where have I seen Issa Rae before? Rae is known for her work as a producer and actress, co-creating the HBO series Insecure. She's also appeared in films like The Hate U Give, The Lovebirds, and Vengeance.
Karan Soni as Pavitr Prabhakar / Spider-Man India
Joining the hoards of spider-people in the new movie is actor Karan Soni as Spider-Man India. He was first introduced in the comics in 2005 and is given his powers by an ancient yogi.
Where have I seen Karan Soni before? Soni is no stranger to the MCU, as he's probably best known for his role as Dopinder in the Deadpool films. He's also appeared in films like Ghostbusters: Answer the Call and Pokemon: Detective Pikachu.
Daniel Kaluuya as Hobart "Hobie" Brown / Spider-Punk
Are we done with Spideys yet? Not even close. Daniel Kaluuya will also rock up as Spider-Punk, a character who was first introduced to the comic books in 2015. In the comics, Hobie Brown is a homeless teenager who gets his powers from a spider after Norman Osborn's toxic waste dumping.
Where have I seen Daniel Kaluuya before? Oscar winner Kaluuya has appeared across a variety of genres, from sci-fi (with Doctor Who and Black Mirror) to horror (with Jordan Peele's Get Out) and drama (with Judas and the Black Messiah). Many UK fans will remember him starting his career with an appearance in teen drama Skins, though.
Oscar Isaac as Miguel O'Hara / Spider-Man 2099
Adding to the list of Spideys is none other than Oscar Isaac, playing Spider-Man 2099. The character first appeared in the comic books in 1992. Miguel O'Hara is introduced as an Irish-Mexican geneticist who attempts to recreate the abilities of the original Spider-Man. As expected, things don't exactly go to plan...
Where have I seen Oscar Isaac before? Isaac has appeared in franchises including Star Wars (as pilot Poe Dameron), X-Men and Dune as Duke Leto Atreides. He's also no stranger to the MCU, having appeared in the series Moon Knight.
Andy Samberg as Ben Reilly / Scarlet Spider
Completing our roster of Spider-People is Andy Samberg as Scarlet Spider. Introduced to the comic books in 1995, he's a genetic replicate of the original Spider-Man.
Where have I seen Andy Samberg before? Samberg is comedy icon, perhaps best known for his role as Jake Peralta in Brooklyn Nine-Nine. He's also a former cast member of Saturday Night Live, a member of the comedy group The Lonely Island, and has appeared in films like Palm Springs.
Jorma Taccone as Vulture
Bringing us a new version of the villainous Vulture is actor Jorma Taccone. Vulture has been brought to life by various actors throughout Spider-Man's cinematic history, most prominently Michael Keaton. Now in animated form, however, he's set to get a whole new lease of life.
Where have I seen Jorma Taccone before? Taccone is also known as a member of The Lonely Island alongside Andy Samberg. He and Samberg joined the cast of SNL together in 2005. He's also appeared in films like Hot Rod and Land of the Lost.
Brian Tyree Henry as Jefferson Davis
Henry is back as Miles's father, Officer Jefferson Davis. It's not known what role he'll have to play in the new film, but we're sure Miles will need his dear dad at some point!
Where have I seen Brian Tyree Henry before? Henry is no stranger to the MCU, playing Phastos in Eternals. He's also known for roles in films like If Beale Street Could Talk, Joker, Bullet Train and Causeway, which earned him an Oscar nomination.
Luna Lauren Vélez as Rio Morales
Of course, Miles's mother Rio is back, as is voice actress Luna Lauren Vélez. She's integral to Miles's story - and just how important she is could be shown in Across the Spider-Verse.
Where have I seen Luna Lauren Vélez before? Vélez has previously appeared in TV shows including Dexter, Ugly Betty and Oz, and films including City Hall alongside Al Pacino.
Greta Lee as Lyla
Hologram Lyla is back to help Miles/Spidey to gather all the information he needs - but as for how she'll deal with the multiverse remains to be seen.
Where have I seen Greta Lee before? Lee is perhaps best known for her role as Soojin in Girls. She's also guest starred in the likes of New Girl and Wayward Pines.
Take part in the Screen Test, a project from Radio Times and the Universities of Sussex and Brighton, to explore the role of television and audio in our lives.