The tour will begin on Monday 17 September at 10:07am when Eunice Gayson, who appeared in Dr No and From Russia With Love, will appear at Eilean Donan Castle in Syle of Lochalsh - which was used in the 1999 film The World Is Not Enough.
Gayson and the golden briefcase will then set off in the Aston Martin which featured in Quantum of Solace to start its 900 mile journey.
The golden case will travel the length of the country stopping off at the most memorable British locations including the Eden Project in Cornwall, which doubled as the diamond mine in Die Another Day, and the Nene Valley Railway, where stunt performers will run along a moving train with the golden briefcase to replicate a scene in Octopussy.
A number of other Bond stars including Shirley Eaton - who was famously painted gold in the film Goldfinger - will make an appearance along the route before former Bond girl Britt Ekland and Bond villain Richard Kiel arrive in central London to deliver the golden briefcase to Oxford Street.
The 23rd Bond film Skyfall, starring current 007 Daniel Craig, is in UK cinemas on 26 October.